Figure 2. p62 deletion was confirmed in lenses of p62 null mice interbred with homozygous Cx50D47A mice. Representative immunoblot shows p62 levels in lens homogenates from 1-month-old homozygous Cx50D47A littermates that were wild-type for p62 (p62^+/+-Cx50^D47A/D47A) or heterozygous (p62^+/−-Cx50^D47A/D47A) or homozygous (p62^−/−-Cx50^D47A/D47A) p62 null. The migration positions of the molecular mass markers are indicated on the left. The graph shows the mean (bar) + standard error of the mean (SEM) of the densitometric values of the immunoreactive bands obtained from three biologic replicates expressed in arbitrary units (a.u.). Asterisks denote statistically significant differences from p62^+/+-Cx50^D47A/D47A values (p<0.05).