**Figure 1. **Levels of total p62 and its phosphorylated forms are altered in lenses of homozygous Cx50D47A mice. **A**–**C**: Representative immunoblots show levels of total p62 (**A**), p62 phosphorylated at threonine-269/serine-272 (P-p62(T269/S272) (**B**), and p62 phosphorylated at serine-349 (P-p62(S349) (**C**) in lens homogenates prepared from 1-month-old wild-type, or heterozygous or homozygous Cx50D47A mice. The migration positions
of the molecular mass markers are indicated on the left. **D**: The graph shows the densitometric values of the immunoreactive bands of total p62. Data are presented as the mean (bar)
+ standard error of the mean (SEM; n = 6 sets) in arbitrary units (a.u.). **E**, **F**: The graphs show the ratio of p62 phosphorylated at T269/S272 to total p62 (**E**) and the ratio of p62 phosphorylated at S349 to total p62 (**F**) calculated from the densitometric values of the immunoreactive bands. Data are presented as the mean (bar) + SEM; n = 6
sets for P-p62(T269/S272), and n = 3 sets for P-p62(S349). Asterisks denote statistically significant differences from wild-type
lenses (p<0.05).