Figure 4 of Hollborn, Mol Vis 2020; 26:188-203.


Figure 4. Receptor-mediated signaling involved in osmotic and hypoxic expression of the OPN gene in RPE cells. The level of OPN mRNA was determined with real-time reverse transcription (RT)–PCR in cells cultured for 24 h in iso- (control; A) and hyperosmotic (+ 100 mM NaCl) media (B), and in the presence of CoCl2 (150 µM; C), respectively. The following agents were tested: the inhibitor of the VEGF receptor-2, SU1498 (10 µM), the inhibitor of the PDGF receptor tyrosine kinase, AG1296 (10 µM), the inhibitor of the EGF receptor tyrosine kinase, AG1478 (600 nM), the inhibitor of TGF-β1 superfamily activin receptor-like kinase receptors, SB431542 (10 µM), the FGF receptor kinase inhibitor, PD173074 (500 nM), the broad-spectrum metalloproteinase inhibitor 1,10-phenanthroline (1,10-Phen; 10 µM), and a human recombinant IL-1 receptor antagonist (IL-1RA; 1 µg/ml). The numbers of independent experiments using cell lines from different donors are indicated in or above the bars. Statistically significant difference versus unstimulated control: *p<0.05. Statistically significant difference versus NaCl control: p<0.05. Statistically significant difference versus CoCl2 control: p<0.05.