Table 2 of
Atilano, Mol Vis 2020; 26:158-172. Table 2 of
Atilano, Mol Vis 2020; 26:158-172.
Table 2. Opsin Primers for PCR and sequencing.
| Symbol | Primer F | Primer R | Internal sequencing primers | Product |
|---|---|---|---|---|
| PRIMERS | size (bp) | |||
| OPN1LWa | GGCAACATAGTGAGACCTCTTCTC | CCAGCAGACGCAGTACGCAAAGAT | E3F: CTCAGTCCGTGGAGCCCTGAATTC | 4045 |
| E4F: ACAAACCCCACCCGAGTTAG | ||||
| E4R: GACTCATTTGAGGGCAGAGC | ||||
| OPN1MWa | GGCAACATAGTGAGACCTCTTCTC | CCAGCAGAAGCAGAATGCCAGGAC | E3F: CTCAGTCCGTGGAGCCCTGAATTC | 4045 |
| E4F: ACAAACCCCACCCGAGTTAG | ||||
| E4R: GACTCATTTGAGGGCAGAGC | ||||
| OPN1SWb | AAGAGGACTCAGAGGGGGGTGTG | AAAATTTAATTCTAGCTGTTGCAAAC | E2F: GCCCATTATCCTCACATTTCACC | 3326 |
| E3F: GGAGAACACAATCCAGGCATCT | ||||
| E4F: GATGTGATGCTTTCCGTGCT | ||||
| RHOc | TCAGAACCCAGAGTCATCCA | CCTACTGTGTGCCCCATTCT | E1R: GGACAGGAGAAGGGAGAAGG | 6715 |
| E2R: CCAGCCCTTGTAGCAACATT | ||||
| E3_4R: ACTGCTGACCCAAGACTGCT | ||||
| E5F: AGCTGGATTTGAGTGGATGG | ||||
| EXON 5 - OPN1LW and OPN1MWd | CTATGCCTGGGTCACCTGCCTC | CTTATCAGAGACATGATTCCAGGTGG | N/A | 398 |