Figure 7. Cellular localization and combination of GCAP1 and retGC1. A: FLAG-GCAP1-wild type (WT)/D144G (red) and green fluorescent protein (GFP)-retGC1 (green) were coexpressed in human embryonic kidney (HEK)-293 cells. B: Interactions between FLAG-GCAP1-WT/D144G and GFP-retGC1 were detected with the co-IP assay. HEK-293 cells were cotransfected with FLAG-tagged GCAP1-WT or FLAG-GCAP1-D144G in the presence or absence of GFP-tagged retGC1. A: The precipitated complexes were probed with the FLAG antibody to detect FLAG-GCAP1-WT/D144G. The cell lysates were probed with the anti-GFP antibody and anti-FLAG antibody in western blotting for analysis of GFP-retGC1 expression (B) and FLAG-GCAP1-WT/D144G (C), respectively. WB, western blotting. IP, immunoprecipitation. The data are representative of three independent experiments.