Figure 1 of Tanaka, Mol Vis 2019; 25:902-911.


Figure 1. Protective effects of PGRN and cleaved PGRN treated with elastase against light-induced photoreceptor cell death. A: Progranulin (PGRN) was cleaved by elastase. Western blotting of recombinant PGRN and recombinant PGRN treated with 0.1, 0.5, and 1.0 Unit/ml elastase from the porcine pancreas. Recombinant PGRN was incubated with elastase for 1 h. The left and right images were detected with long and short exposure times, respectively. B: Representative images of cone photoreceptor cells (661W) stained with Hoechst 33,342 and propidium iodide (PI). C: 661W cells were incubated under light exposure. PBS, PGRN, cleaved PGRN ([P + E] + phenylmethylsulfonyl fluoride [PMSF]), PMSF, and elastase incubated with PMSF were added to the medium. The number of PI-positive cells was increased after light exposure. Recombinant PGRN and cleaved PGRN statistically significantly suppressed light-induced photoreceptor cell death. Data are shown as mean ± standard error of the mean (SEM; n = 5). ##, p<0.01 versus control; **, p<0.01 versus vehicle *, p<0.05 versus vehicle (Tukey’s test). P, PGRN; E, elastase. Scale bar = 100 µm.