Figure 1. Effects of oral administration of crocetin on retinal thickness in the murine RVO model. A: Experimental protocol in vivo with oral administration of crocetin. Retinal vein occlusion (RVO) mice were orally administered crocetin 1 and 6 h before laser occlusion and immediately, 6 h, 12 h, and 18 h after laser irradiation. Retinal thickness was examined 24 h after laser irradiation. B: Representative images of hematoxylin and eosin (H&E) staining in the control, vehicle-treated, and crocetin-treated groups 24 h after laser irradiation. Scale bar indicates 50 µm. C: Quantification of the thickness of the inner nuclear layer (INL). The thickness of the INL was statistically significantly increased 1 day after laser irradiation, and this increase was improved with the crocetin treatment. Data are shown as mean ± standard error of the mean (SEM; n=5 or 6). ^#p<0.05, ^##p<0.01 (versus control group; Student t test). *p<0.05, **p<0.01 (versus vehicle group; Student t test). INL, inner nuclear layer; ONL, outer nuclear layer.