Figure 5. MEL activates AKT/FoxO1 and ERK1/2 only in the presence of the MT_1/2h. A: Effect of melatonin (MEL; 100 nM) or IIK7 (1,000 nM) 90-min treatment on the phosphorylation of AKT and FoxO1 in 661W cells and 661W-melatonin receptor type 2(MT₂)^−/− cells. B: Effect of MEL (100 nM) or IIK7 (1,000 nM) 90-min treatment on the phosphorylation of ERK1/2 in 661W cells and 661W-MT₂^−/− cells. In all cases, results (n = 5) are shown as mean ± standard error of the mean (SEM). Asterisks indicate statistically significant differences compared to the control group with one-way ANOVA (Student–Newman–Keuls (SNK) post-hoc test; p<0.05).