Figure 3 of Sánchez-Bretaño, Mol Vis 2019; 25:791-799.


Figure 3. Genetic ablation of MT2 with CRISPR/Cas9 system does not affect MT1 mRNA or protein. Top panels: cDNA from 661W and 661W-MT2−/− cells was analyzed with reverse transcriptase PCR (RT–PCR) to detect MT1 (left) and MT2 (right) expression. Middle panels: Immunofluorescence was performed in 661W and 661W-melatonin receptor type 2 (MT2)−/− cells to detect MT1 (left) and MT2 (right) proteins. The positive signal of each receptor is shown in green, and nuclei are counterstained in red. Lower panel: The presence or absence of MT1/MT2 heterodimer in 661W and 661W-MT2−/− cells was assessed with proximity ligation assay (PLA; positive signal in red), and nuclei were counterstained in green. MT1 expression (transcript and protein) was detected in 661W and 661W-MT2−/− cells, whereas MT2 expression (transcript and protein) was detected only in 661W cells. Accordingly, MT1/2h was found only in 661W cells. Scale bars = 100 µm.