Figure 6. Glutathione peroxidase 3–antisense (GPX3-AS) inhibits H₂O₂-induced SRA01/04 cell apoptosis by upregulating GPX3. A, B: SRA01/04 cells were transfected with scramble or GPX3 siRNA, and GPX3 mRNA and GPX3-AS levels were detected by quantitative real-time PCR (qRT-PCR). **p<0.01, ***p<0.001 versus scramble siRNA group. C: pcDNA-GPX3-AS and scramble siRNA or GPX3 siRNA were cotransfected into SRA01/04 cells. GPX3 mRNA levels were detected by qRT-PCR. ***p<0.001 versus pcDNA GPX3-AS plus scramble group. D, E: SRA01/04 cells were transfected with pcDNA plus scramble siRNA, pcDNA plus GPX3 siRNA1, pcDNA-GPX3-AS plus scramble siRNA or pcDNA-GPX3-AS plus GPX3 siRNA1 for 24 h, respectively. Then, they were exposed to H₂O₂ for 24 h. Apoptotic cells were detected by flow cytometry. *p<0.05 versus pcDNA plus scramble siRNA group; ***p<0.001 versus pcDNA-GPX3-AS plus scramble siRNA group. n = 5 per group.