Figure 4. Glutathione peroxidase 3–antisense (GPX3-AS) inhibits H₂O₂-induced SRA01/04 cell apoptosis. SRA01/04 cells were transfected with AS oligo (ASO)-NC, ASO, pcDNA, or pcDNA-GPX3-AS for 24 h, and then the cells were exposed to H₂O₂ for an additional 24 h. A, D: Quantitative real-time PCR (qRT-PCR) was performed to detect GPX3-AS levels. (A) *p<0.05, ***p<0.001 versus ASO-NC group. (D) ***p<0.001 versus pcDNA group. B, E: Cell viability was detected by Cell Counting Kit-8 (CCK-8) assay. (B) * p<0.05 versus ASO-NC group. (E) ***p<0.001 versus pcDNA group. C, F: Apoptotic cells were analyzed by TUNEL staining. Scale bar = 50 μm. G, H: Flow cytometry analysis of SRA01/04 cell apoptosis. **p<0.01 versus ASO-NC group. ***p<0.001 versus pcDNA group. n = 5 per group.