Figure 3. Decreased glutathione peroxidase 3–antisense (GPX3-AS) expression and increased human lens epithelial cell (HLEC) apoptosis are induced by H₂O₂. A: SRA01/04 cells were treated with H₂O₂ for 24 h, and the GPX3-AS levels were detected by quantitative real-time PCR (qRT-PCR). **p<0.01 versus control group. B: Cell viability was detected by Cell Counting Kit-8 (CCK-8) assay. ***p<0.001 versus control group. C: TUNEL staining of SRA01/04 cells with or without H₂O₂ treatment. Scale bar = 50 μm. D, E: Active caspase-3, Bax, and Bcl-2 expression in SRA01/04 cells was detected by western blot. *p<0.05 versus each control group. F, G: Flow cytometry analysis of H₂O₂-induced apoptosis. ***p<0.001 versus control group. n = 5 per group.