Figure 2. Glutathione peroxidase 3–antisense (GPX3-AS) is mainly localized in SRA01/04 cell nuclei. A: The subcellular localization of GPX3-AS in SRA01/04 cells was detected by fluorescence in situ hybridization (FISH). U6 was used as a representative for nuclear localization, and 18S was used as a representative for cytoplasmic localization. B: Nuclear and cytoplasmic RNA isolation showed that GPX3-AS was mainly localized in the nuclei of SRA01/04 cells. The U6 and 18S mRNAs were selected as the nuclear and cytoplasmic control transcripts, respectively. n = 5 per group.