Figure 1 of Tu, Mol Vis 2019; 25:734-744.


Figure 1. Long noncoding RNA (lncRNA) glutathione peroxidase 3–antisense (GPX3-AS) is decreased and apoptosis-related proteins are increased in the anterior lens capsules of age-related cataract (ARC) tissue. A: A heat map sequence analysis shows differentially expressed lncRNAs in the anterior lens capsules of transparent (n = 5) and ARC lenses (n = 6). B: GPX3-AS levels in the anterior lens capsules of the normal and ARC groups were detected by quantitative real-time PCR (qRT-PCR). *p<0.05 versus normal group. C: GPX3-AS levels in the normal, age-related cortical cataract (ARCC), age-related nuclear cataract (ARNC), and age-related posterior subcapsular cataract (ARPC) groups were detected by RT-PCR. *p<0.05 versus normal group. D: Anterior lens capsules were stained for active caspase-3 (red). The nuclei were stained blue with 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bar = 100 μm. E, F: Active caspase-3, BCL2 associated X(Bax), B-cell lymphoma-2(Bcl-2) expression in the anterior lens capsules of normal and cataract tissues was detected by western blot. *p<0.05 versus each normal group. n = 6 per group.