Figure 4. miR-30b mimic modulates the axon and dendrite attributes of RGCs. RGCs transfected with miR-30b mimic, inhibitor, or miRNA NC were challenged with rAAV. A: Immunostaining of RGC cultured with miR-30b mimic and miRNA NC for 120 h. Scale bar, 20 μm. (mean ± SD; 44-45 cells per treatment; *p<0.05, compared to miRNA NC; #p<0.05, compared to the alternative dose). B: The longest axon and dendrite per RGC were measured after culture of postnatal RGCs after 120 h (mean ± SD; n=3 cultures; 44-45 cells per treatment; *p<0.05 compared to miRNA NC; #p<0.05, compared to the alternative dose). C, D: The numbers of cells of different numbers of axons and dendrites were measured in RGCs transfected with miR-30b mimic, inhibitor, or miRNA NC after 120 h (44-45 cells per treatment, *p<0.05). E: The percentage of bipolar cells was measured in RGCs transfected with miR-30b mimic, inhibitor, or miRNA NC after 120 h (95-85 cells per treatment). F: Correlations between average axon length and average dendrite length of RGCs (44-45 cells per treatment, *p<0.05).