Figure 5. The Sox9 knockdown attenuated the upregulation of GFAP, vimentin, nestin, and Cspg protein levels, as measured by western blot analysis. A: Western blot analysis was used to measure the GFAP, vimentin, nestin, and Cspg protein levels in the retinas from the control and Sox9-shRNA groups on days 3, 7, and 14 after light expose. Β-actin served as the loading control. The relative expressions of GFAP (B), vimentin (C), nestin (D), and Cspg (E) protein levels in Sox9-shRNA group are significantly downregulated compared with the control group. Data are expressed as a percentage of the control values presented as mean ± SD (n = 3), t test, *p<0.05.