Figure 7 of Minogue, Mol Vis 2019; 25:535-545.


Figure 7. Deletion of Chop does not affect levels of proteins associated with different intracellular compartments that are not completely removed during cell differentiation in Cx50D47A lenses. AC: Immunoblots of histone H3 (H3; A, top panel), translocase of outer mitochondrial membrane 20 (TOM20; B, top panel) and calreticulin (CALR; C, top panel) in lens homogenates prepared from 1-month-old Cx50D47A homozygotes that were wild type for Chop (Chop+/+) or heterozygous (Chop+/−) or homozygous (Chop−/−) Chop knockout. The migration positions of the molecular mass markers are indicated on the left. Graphs in the bottom panels show the densitometric quantification of the H3 (A), TOM20 (B), and CALR (C) immunoreactive bands. Data are presented as the mean (bar) + standard error of the mean obtained in three independent biological replicates.