Appendix 7 of Zhang, Mol Vis 2019; 25:462-476.


Appendix 7.

Figure showing full-sized images that allow comparison of fundus autofluorescent spots observed in vivo with Iba-1 immuno-signal in post-mortem RPE flatmount from the same eye. In vivo fundus examination at the level of the subretinal space was conducted one week following induction of the I307N Rho degeneration, followed by euthanization and harvest of eyes for subsequent fixation and immunohistochemical confocal microscopy on RPE flatmounts. A: Uninduced eyes showed scant blue autofluorescence at the level of the subretinal space in vivo (left) and RPE flatmouns of those same eyes showed little Iba-1 immuno-signal (right). B: In eyes of induced I307N Rho mice housed with inactive running wheels, numerous and widespread autofluorescent spots were observed in vivo (left) and matching flatmounts exhibited numerous Iba-1-positive cells (right). C: Exercised mice that had undergone degeneration induction showed greatly diminished numbers of autofluorescent spots (left) and Iba-1-positive cells (right). Overall patterns of signal appear similar in paired fundus and flatmount images, suggesting the possibility that some autoflourescent spots observed via in vivo fundus imaging are likely to be overexpressing Iba-1 and may be microglia or other inflammatory cells. To access the data, click or select the words “Appendix 7.”