Table 1 of
Zhuang, Mol Vis 2019; 25:427-437. Table 1 of
Zhuang, Mol Vis 2019; 25:427-437.
Table 1. The PCR primers and conditions for crystallin genes.
| Gene | Entrez Gene ID | #OMIM | Exon | Primer name | Primer sequence(5'-3') | Amplicon size (bp) | PCR condition* |
|---|---|---|---|---|---|---|---|
| CRYAA (NM_000394) | 1409 | 123580 | 1 | CRYAAe1F | CTTCTTCATGAGCTCACGCC | 443 | 59 °C |
| CRYAAe1R | TGACGGAGCAAGACCAGAGT | ||||||
| CRYAA (NM_000394) | 1409 | 123580 | 3 | CRYAAe3F | CAGGCTGAGGTCCAGAGAAG | 510 | 60 °C/GC Buffer1 |
| CRYAAe3R | GGGAAGCAAAGGAAGACAGA | ||||||
| CRYAB (NM_001289807) | 1410 | 123590 | 1 | CRYABe1F | AACCCCTGACATCACCATTC | 352 | 58 °C |
| CRYABe1R | AAGGACTCTCCCGTCCTAGC | ||||||
| CRYAB (NM_001289807) | 1410 | 123590 | 3 | CRYABe3F | GTTGTCATGGCATTTGGTCTC | 433 | 58 °C |
| CRYABe3R | CTTGATAATTTGGGCCTGCC | ||||||
| CRYBA1 (NM_005208) | 1411 | 123610 | 3 | CRYBA1e3F | ACTCTGGGCAAATGAACACC | 399 | 58 °C |
| CRYBA1e3R | TCCCCTATCCCCACTCTATG | ||||||
| CRYBA1 (NM_005208) | 1411 | 123610 | 4 | CRYBA1e4F | CCTGTCAACTCATTCCTCAACTC | 493 | 58 °C |
| CRYBA1e4R | CACCTGGTGGAGAAAAATCAA | ||||||
| CRYBA4 (NM_001886) | 1413 | 123631 | 4 | CRYBA4e4F | CTCCCCTAGTCGTGACAACC | 394 | 58 °C |
| CRYBA4e4R | TTTCAACTCTGGAACCTTTGA | ||||||
| CRYBB1 (NM_001887) | 1414 | 600929 | 6 | CRYBB1e6F | GCACAGAGCAGGAAGGGATA | 498 | 58 °C |
| CRYBB1e6R | CGAGGAAGTCACATCCCAGT | ||||||
| CRYBB2 (NM_000496) | 1415 | 123620 | 2 | CRYBB2e2F | CCTTCAGCATCCTTTGGGTTCTCT | 597 | 58 °C |
| CRYBB2e2R | GCAGTTCTAAAAGCTTCATCAGTC | ||||||
| CRYBB2 (NM_000496) | 1415 | 123620 | 5 | CRYBB2e5F | TGGGTGCACTGGGAAGAGA | 399 | 58 °C/GC Buffer1 |
| CRYBB2e5R | GAAGCCAGAGGTCAGCAGAG | ||||||
| CRYBB2 (NM_000496) | 1415 | 123620 | 6 | CRYBB2e6F3 | CTGACCCCAGTACAGTACAGT | 661 | 61 °C |
| CRYBB2e6R3 | CATTTCTCTCTCGCTGTCACTCTCTC | ||||||
| CRYBB3 (NM_004076) | 1417 | 123630 | 6 | CRYBB3e6F | GAGGAATGTAGGCAGGCAGA | 480 | 58 °C |
| CRYBB3e6R | TCCTTCAGCACGCCTCTC | ||||||
| CRYGC (NM_020989) | 1420 | 123680 | 1, 2 | CRYGCe1/2F | TGCATAAAATCCCCTTACCG | 556 | 58 °C |
| CRYGCe1/2R | CCTCCCTGTAACCCACATTG | ||||||
| CRYGC (NM_020989) | 1420 | 123680 | 3 | CRYGCe3F | CGCAGCAACCACAGTAATCT | 579 | 58 °C |
| CRYGCe3R | CCCACCCCATTCACTTCTTA | ||||||
| CRYGD (NM_006891) | 1421 | 123690 | 1, 2 | CRYGDe1/2F | AGAACACGAAAATGCCCTTG | 579 | 58 °C/GC Buffer1 |
| CRYGDe1/2R | TGCTTGAAACCATCCAGTGA | ||||||
| CRYGD (NM_006891) | 1421 | 123690 | 3 | CRYGDe3F | CCTCACCAAGCTGGACTGC | 421 | 58 °C |
| CRYGDe3R | GCCAGGAACACACAGAAAATATT | ||||||
| CRYGS (NM_017541) | 1427 | 123730 | 2 | CRYGSe2F | GAAACCATCAATAGCGTCTAAATG | 229 | 58 °C |
| CRYGSe2R | TGAAAAGCGGGTAGGCTAAA | ||||||
| Gene | Entrez Gene ID | #OMIM | Exon | Primer name | Primer sequence(5'-3') | Amplicon size (bp) | PCR condition* |
| CRYAA (NM_000394) | 1409 | 123580 | 1 | CRYAAe1F | CTTCTTCATGAGCTCACGCC | 443 | 59 °C |
| CRYAAe1R | TGACGGAGCAAGACCAGAGT | ||||||
| CRYAA (NM_000394) | 1409 | 123580 | 3 | CRYAAe3F | CAGGCTGAGGTCCAGAGAAG | 510 | 60 °C/GC Buffer1 |
| CRYAAe3R | GGGAAGCAAAGGAAGACAGA | ||||||
| CRYAB (NM_001289807) | 1410 | 123590 | 1 | CRYABe1F | AACCCCTGACATCACCATTC | 352 | 58 °C |
| CRYABe1R | AAGGACTCTCCCGTCCTAGC | ||||||
| CRYAB (NM_001289807) | 1410 | 123590 | 3 | CRYABe3F | GTTGTCATGGCATTTGGTCTC | 433 | 58 °C |
| CRYABe3R | CTTGATAATTTGGGCCTGCC | ||||||
| CRYBA1 (NM_005208) | 1411 | 123610 | 3 | CRYBA1e3F | ACTCTGGGCAAATGAACACC | 399 | 58 °C |
| CRYBA1e3R | TCCCCTATCCCCACTCTATG | ||||||
| CRYBA1 (NM_005208) | 1411 | 123610 | 4 | CRYBA1e4F | CCTGTCAACTCATTCCTCAACTC | 493 | 58 °C |
| CRYBA1e4R | CACCTGGTGGAGAAAAATCAA | ||||||
| CRYBA4 (NM_001886) | 1413 | 123631 | 4 | CRYBA4e4F | CTCCCCTAGTCGTGACAACC | 394 | 58 °C |
| CRYBA4e4R | TTTCAACTCTGGAACCTTTGA | ||||||
| CRYBB1 (NM_001887) | 1414 | 600929 | 6 | CRYBB1e6F | GCACAGAGCAGGAAGGGATA | 498 | 58 °C |
| CRYBB1e6R | CGAGGAAGTCACATCCCAGT | ||||||
| CRYBB2 (NM_000496) | 1415 | 123620 | 2 | CRYBB2e2F | CCTTCAGCATCCTTTGGGTTCTCT | 597 | 58 °C |
| CRYBB2e2R | GCAGTTCTAAAAGCTTCATCAGTC | ||||||
| CRYBB2 (NM_000496) | 1415 | 123620 | 5 | CRYBB2e5F | TGGGTGCACTGGGAAGAGA | 399 | 58 °C/GC Buffer1 |
| CRYBB2e5R | GAAGCCAGAGGTCAGCAGAG | ||||||
| CRYBB2 (NM_000496) | 1415 | 123620 | 6 | CRYBB2e6F3 | CTGACCCCAGTACAGTACAGT | 661 | 61 °C |
| CRYBB2e6R3 | CATTTCTCTCTCGCTGTCACTCTCTC | ||||||
| CRYBB3 (NM_004076) | 1417 | 123630 | 6 | CRYBB3e6F | GAGGAATGTAGGCAGGCAGA | 480 | 58 °C |
| CRYBB3e6R | TCCTTCAGCACGCCTCTC | ||||||
| CRYGC (NM_020989) | 1420 | 123680 | 1, 2 | CRYGCe1/2F | TGCATAAAATCCCCTTACCG | 556 | 58 °C |
| CRYGCe1/2R | CCTCCCTGTAACCCACATTG | ||||||
| CRYGC (NM_020989) | 1420 | 123680 | 3 | CRYGCe3F | CGCAGCAACCACAGTAATCT | 579 | 58 °C |
| CRYGCe3R | CCCACCCCATTCACTTCTTA | ||||||
| CRYGD (NM_006891) | 1421 | 123690 | 1, 2 | CRYGDe1/2F | AGAACACGAAAATGCCCTTG | 579 | 58 °C/GC Buffer1 |
| CRYGDe1/2R | TGCTTGAAACCATCCAGTGA | ||||||
| CRYGD (NM_006891) | 1421 | 123690 | 3 | CRYGDe3F | CCTCACCAAGCTGGACTGC | 421 | 58 °C |
| CRYGDe3R | GCCAGGAACACACAGAAAATATT | ||||||
| CRYGS (NM_017541) | 1427 | 123730 | 2 | CRYGSe2F | GAAACCATCAATAGCGTCTAAATG | 229 | 58 °C |
| CRYGSe2R | TGAAAAGCGGGTAGGCTAAA |