Figure 8. The effects of 5-Aza-dC on the promoter activity of COL1A2. A: The time course of the experiment. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days. The cells were then passaged, and plasmids containing the COL1A2 promoter were introduced. After 24 h of serum starvation, the cells were treated with transforming growth factor (TGF)-β2 (2.5 ng/ml or 5.0 ng/ml) for 24 h, and subjected to a luciferase assay. B, C: The transcriptional activity was assessed with the transient transfection of a luciferase reporter gene fused with the COL1A2 promoter, and the activity relative to an internal control was calculated (n=4); *p<0.05. Bars indicate the standard error.