Figure 7. The effects of 5-Aza-dC on time-dependent changes in Smad3 and Akt. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days. The cells were then passaged, and treated with transforming growth factor (TGF)-β2 for 2 days. The cells were then subjected to western blotting. The protein bands were quantified, and the ratios of phospho-Smad3 to total Smad3 (A), and phospho-Akt to total Akt (B), were calculated (n=3 and 4, respectively); *p<0.05. Bars indicate the standard error.