Figure 6. The effects of 5-Aza-dC on Smad3. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days.
The cells were then passaged, and treated with transforming growth factor (TGF)-β2 for 2 days. The cells were then subjected
to western blotting. The protein bands were quantified, and the ratios of total Smad3 to β-actin (A), and phosphorylated Smad3 to total Smad3 (B), were calculated (n=4); *p<0.05. Bars indicate the standard error.