Figure 5. The effect of 5-Aza-dC on cell death. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days. After passage, the cells were treated with transforming growth factor (TGF)-β2 for 2 days. A: The cells were stained with Hoechst 33,342 and PI. Cells stained with Hoechst and PI were considered dead cells (n=4). Cells treated with 4 mM H₂O₂ for 2 h were used as a positive control. B: The cells were subjected to lactate dehydrogenase (LDH) assay (n=4). Bars indicate the standard error.