Figure 4. The effect of 5-Aza-dC on cell proliferation. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days. The cells were then passaged and treated with transforming growth factor (TGF)-β2 for 2 days. A: The cells were stained with Hoechst 33,342 and propidium iodide (PI). The number of live cells (Hoechst-positive, PI-negative) was counted (n=4). B: The cells were subjected to the WST-8 assay (n=4); *p<0.05. Bars indicate the standard error.