Figure 3. The effect of 5-Aza-dC on the expression of mRNA coding fibrogenic genes. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days. After passage, the cells were treated with transforming growth factor (TGF)-β2 for 2 days, and then subjected to real-time reverse transcription-PCR (RT–PCR). The expression levels of ACTA2 (A), FN1 (B), and COL1A1 (C) were calculated (n=4); *p<0.05. Bars indicate the standard error.