Figure 1. The effect of 5-aza-2'-deoxycytidine (5-Aza-dC) on α-SMA expression. A: Time course of the experiment. Primary human conjunctival fibroblasts were pretreated with 5-Aza-dC once daily for 2 days.
After passage, the cells were treated with transforming growth factor (TGF)-β2 for 2 days, and then subjected to western blotting.
B: The protein bands were quantified, and the ratios of α-smooth muscle actin (SMA) to β-actin were calculated (n=5); *p<0.05.
Bars indicate the standard error.