Figure 6. Minocycline attenuates LPS-induced retinal inflammation via suppression of microglia activation. A: Immunofluorescent staining of retinal whole-mounts show iba-1 (red) at 3 h post-infection, and interleukin-1 beta (IL-1β; green) and chemokine (C-C motif) ligand 2 (CCL-2; green) at 24 h in the lipopolysaccharide (LPS) stimulation with or without minocycline treatment eyes (n=4 per group). Scale bar: 40 μm. B: Real-time PCR analysis of the mRNA expression of tumor necrosis factor-α (TNF-α), CCL-2, IL-1β, and IL-6 in LPS stimulation with or without minocycline treatment eyes at 24 h post-infection. *p<0.05 compared with the LPS treated with PBS group.