Figure 5. Minocycline inhibits LPS-induced the proinflammatory response of microglia in vitro.A: Real-time PCR analysis of IL-1β, CCL-2, TNF-α, and IL-6 mRNA expression in BV-2 cells exposed to 100 ng/ml of lipopolysaccharide (LPS) with or without minocycline (50 μg/ml) for 6 h. *p<0.05 compared with the LPS plus PBS group. B: Immunofluorescent staining analysis of IL-1β (green) and CCL-2 (red) expression in BV-2 microglial cells exposed to 100 ng/ml of LPS with or without minocycline (50 μg/ml) for 6 h (n=3 per group). Scale bars: 40 μm.