Figure 4. Microglia can be rapidly activated and peripheral leukocytes were recruited to the retina in EIU. A: One microgram of lipopolysaccharide (LPS) was injected into the vitreous of the 4Di-10ASP labeling rats to induce endotoxin-induced uveitis (EIU). The retinas were harvested and stained with a microglial marker CD11b (red) after infection for 3, 6, and 12 h. Representative immunofluorescence images of the retinal whole-mounts were obtained with confocal microscopy (n=6 per group). Scale bars: 40 μm. B: Twenty-four hours after the LPS injection, the retinas were harvested, and real-time PCR was used to analyze the mRNA expression of the chemotactic factor CCL-2, and proinflammatory cytokines TNF-α, IL-1β, and IL-6 (n=5 per group). *p<0.05 compared with the normal control retinas.