Figure 4. Western immunoblot analysis of STAT phosphorylation. Western immunoblot analysis of signal transducer and activator of transcription (STAT)-1 Tyr701, STAT-3 Ser727, STAT-3 Tyr705, and STAT-5 Tyr694 phosphorylation in (A) normal human trabecular meshwork cells or (B) normal human dermal fibroblasts treated with the indicated interleukins for 15 min. Total STATs were used as loading controls. The arrow points to the upper phosphorylated band. C: Densitometry of the phosphorylated Ser727 STAT-3 band. Trabecular meshwork, n=6 technical replicates using four independent cell strains; dermal fibroblasts, n=3 technical replicates from three cell strains. * p<0.05; ** p<0.005 with one-way ANOVA.