Figure 5. Immunohistochemistry staining of the GJA1, GJA8, GJB6, and GJC1 proteins in human anterior segment tissues. GJA1 (red), GJA8 (red), GJB6 (green), and GJC1 (green) are localized in the iris, ciliary body, and the trabecular meshwork with immunohistochemistry (IHC) staining. In the high-magnification images (upright panels), positive staining is shown along the trabecular beams. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Bar = 50 µm.