Molecular Vision: Faralli, Mol Vis 2019; 25:237-254. Table 2

Table 2. DEX-induced changes in genes involved in phagocytosis.

Gene Name	Protein Name	Function in Phagocytosis	FC HTM1	FC HTM2	FC in Rozsa et al # (21)
		Receptors
CD36	CD36 or Thrombospondin receptor	class B scavenger receptor of pathogens, thrombospondin, collagen, lipoproteins, etc	-10.2‡	-5.3†	NR
SIRPA  (SHPS1)	Signal regulatory protein alpha or Tyrosine-protein phosphatase non-receptor type substrate 1	Don’t eat me signal; binds CD47	-1.6*	NC	NR
ITGB3	β3 integrin subunit	Binds ECM proteins; signals to regulate cytoskeleton	4.8‡	6.7‡	3.9
		GTPases, GEFs, GAPs
ABR	Active breakpoint cluster region-related protein	Dual GEF/GAP Negative regulator of phagocytosis	-2.3	-1.6	-1.4
CHN 1 (ARHGAP2)	N-Chimaerin or α-chimaerin	GTPase activating protein (GAP) for Rac1	+3.0	+3.0	1.5
		Adaptors
GULP1	PTB domain-containing engulfment adapter protein 1	Regulates engulfment, coordinates Rac1 activity	-2.9	-3.6	-2.5
		Kinases
PIK3R1	Phosphoinositide-3-kinase regulatory subunit 1	Phosphorylates phosphatidylinositol; involved in integrin, GPCR, RTK and MAPK signaling	4.1‡	4.3‡	2.8
		Ligands
MFGE8	Lactadherin or Milk fat globule-EGF factor 8 protein	Eat-me signal; binds β3 and β5 integrin	2.0*	2.0‡	3.3

Two separate HTM1 (N27TM-2) and HTM2 (N27TM-4) cell strains isolated from the same 27 year old female donor eye were used for this study. The fold change (FC) for HTM1 and HTM2 is the average of 5 and 3 biological replicates, respectively. Data for CD36, β3 integrin and PIK3R1 were previously published (19). Abbreviations: NR, not reported. NC, no change detected. * p< 0.05, † p < 0.01, ‡ p < 0.001, #only genes with a p value <0.005 were included.