Figure 5. FH inhibits the selenite-induced decrease in Nrf-2 and HO-1 in the lens. A: Gene expression levels of Nrf-2 and HO-1 were analyzed with real-time polymerase chain reaction and normalized to glyceraldehyde 3-phosphated dehydrogenase (GAPDH) in the lens. The transcript level represents the mean ± standard deviation (SD) of three independent experiments (n=4). B: The protein level of Nrf-2 was measured in the nuclear fraction of the lens, and the protein level of HO-1 was measured in the cytosol fraction of the lens using western blotting. The protein level represents the mean ± standard deviation (SD) of three independent experiments (n=4). ##p<0.01, ###p<0.001 statistically significant different from CTL. *p<0.05, **p<0.01 statistically significant different from Se. CTL: normal control, Se: selenite-treated, FH80: selenite/FH-treated (80 mg/kg), Cur80: selenite/curcumin-treated (80 mg/kg).