Figure 2. Effects of FH on soluble and insoluble proteins and α-, β-, and γ-crystallins in the lens. (A) Soluble and (B) insoluble proteins of lenses were analyzed in all groups. The results were represented as the mean ± standard deviation (SD) of three independent experiments (n=6). C: The gene expression of the α-, β-, and γ-crystallins were analyzed using real-time polymerase chain reaction, and then normalized to glyceraldehyde 3-phosphated dehydrogenase (GAPDH). The transcript levels were expressed as the mean ± standard deviation (SD) of three independent experiments (n=4). #p<0.05, ##p<0.01, ###p<0.001 statistically significant different from CTL. *p<0.05, **p<0.01, ***p<0.001 statistically significant different from Se. CTL: normal control, Se: selenite-treated, FH40/80/120: selenite/FH-treated (40, 80, 120 mg/kg, respectively), Cur80: selenite/curcumin-treated (80 mg/kg).