Figure 1. To characterize the retinal proteome/phosphoproteome in its entirety, we performed several qualitative mass spectrometry (MS) runs from eight male and eight female mice; pooling the retinas from each animal yielded a total of 32 retinas for each sample, with three technical repeats per sample. Following the initial MS analysis, 10-ul sample aliquots were pooled together to perform phosphopeptide-enrichment procedures. Upon acquisition of the MS data, Ingenuity Pathway Analysis (IPA) software was used to perform bioinformatic analysis.