Table 2 of Maxia, Mol Vis 2018; 24:853-866.

Table 2. Primary antibodies used in the study.

Target Primary antibody Biotinylated secondary antibody
vendor origin dilution incubation antigen retrieval RRID*
PDGFRa R&D Systems Goat (pc) 1:200 O.N. Heat-induced1 AB_354459 Horse anti-goat IgG6
CD34 Santa Cruz Biotechnology Mouse (QBEnd/10) 1:100 1 h. RT n.r. AB_629044 Horse anti-mouse IgG6
c-kit DAKO Rabbit (pc) 1:200 1 h. RT Heat-induced2 AB_2335702 Goat anti-rabbit IgG6
Nestin Novus Biologicals Mouse (10C2) 1:1000 1 h. RT n.r. AB_10001441 Horse anti-mouse IgG6
Vimentin Millipore Goat (pc) 1:2500 1 h. RT n.r. AB_90774 Horse anti-goat IgG6
α-SMA Abcam Rabbit (pc) 1:500 1 h. RT n.r. AB_2223021 Goat anti-rabbit IgG6
Laminin Novus Biologicals Rabbit (pc) 1:500 O.N. Proteolytic3 AB_10001146 Goat anti-rabbit IgG6
S-100 DAKO Rabbit (pc) 1:1000 1 h. RT Proteolytic3 AB_10013383 Goat anti-rabbit IgG6
VEGF Santa Cruz Biotechnology Rabbit (pc) 1:500 O.N. Proteolytic4 AB_2212984 Goat anti-rabbit IgG6
CD133 Abcam Rabbit (pc) 1:1000 1 h. RT Proteolytic4 AB_470302 Goat anti-rabbit IgG6
CD31 DAKO Mouse (JC70A) 1:50 1 h. RT Heat-induced5 AB_2114471 Horse anti-mouse IgG6

Pc, polyclonal. mc, monoclonal. O.N., overnight. RT, room temperature. n.r., not required. Water bath heating-based antigen retrieval at 95 °C in 1TRIS 10mM + EDTA 1mM (pH 9.0) for 20 min; 2citrate buffer, 10mM (pH 6.0) for 30 min; 5TRIS 0.5M (pH 10.0) for 30 min. Proteolytic pre-treatment with Trypsin (Difco, Becton, Dickson and Company, MD), 0.1% 37 °C for 5 min3 or 10 min4. 6Vector Laboratories, Burlingame, CA. *The Resource Identification Initiative (Bandrowski et al. 2015); Resource Identification Portal.

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