Figure 1. The effect of the OCRL D655V mutation on Rab binding. A: Structure of ORCL-Ash domain (green) with Rab8a (purple). L605 is critical for interaction with Rab8a. Residues involved in hydrogen bond interactions within the β5-β6 loop are shown as sticks, including D655. Intercations are shown in dashed lines. B. superposition of the structures of OCRL-Rab8a (3QBT in green) and OCRL-SES1 (3QIS in purple), showing the change in the position of K604 and L605.