Figure 2. YAP siRNA IP injection reduces VEGF expression and the leakage area of CNV. A: YAP and VEGF protein levels after scramble siRNA and YAP siRNA intraperitoneal (IP) injection and intravitreal ranibizumab injection (IVR) were detected with western blotting. GAPDH was used as a loading control. B: The histogram shows the densitometric analysis of the average levels of YAP and VEGF relative to GAPDH. *p<0.05; compared to the 7 day post-laser photocoagulation group. C: Hyper-fluorescence surrounding the laser spots reflects relatively weak leakage (grade 1) in the YAP siRNA IP injected mouse retinas. D: Mouse RPE-choroid flat mount preparations from laser-injured regions were fluorescently labeled with the endothelial and microglial cell marker isolectin-B4 (63X magnification). Scale bars = 100 μm. E: Comparison of choroidal neovascularization (CNV) size in the 7 day post-laser photocoagulation, vehicle, scramble siRNA, YAP siRNA, and RBZ groups. *p<0.05, compared to the 7 day post-laser photocoagulation group.