Figure 3. Gene expression analysis of novel identified PEX material proteins in the lens epithelium. Reverse transcription-PCR (RT–PCR)
was performed on normal lens epithelium mRNA using gene-specific primers (Appendix 1). The resulting PCR products were analyzed
with agarose gel electrophoresis. The products of the expected sizes (see Appendix 1) were observed for the HB (subunit A), HSPB1, and PRDX2 genes but were undetectable for the APOA1, APOA4, and FG (subunits A, B, and C) genes. However, the expected sized products for APOA1, APOA4, and FG subunits were observed in the positive control tissues (blood or placenta). The sizes of the molecular weight standards in
base pairs (bp) are indicated. LC, lens capsule; RT+, with reverse transcriptase; RT-, without reverse transcriptase; neg, negative control.