Appendix 7 of Sharma, Mol Vis 2018; 24:801-817.

Appendix 7. Immunohistochemical labeling of HSPB1 in PEX affected and unaffected lens capsules.

Sections of PEX affected (left panel) and unaffected (right panel) lens capsules from cataract patients were immunolabeled with the anti-HSPB1 antibody. HSPB1-positive labeling (brown) can be seen in the lens epithelium in unaffected lens capsule (right panel) indicating presence of the protein but very weak labeling of PEX material accumulated on affected lens capsule was observed (left panel). Lens capsule in the right hand image is folded over. Representative images from independent experiments on three PEX affected and four unaffected lens capsules are shown. Images are at 60× original magnification. To access the data, click or select the words “Appendix 7.”