Appendix 2 of Lin, Mol Vis 2018; 24:789-800.
Nintedanib suppresses Smad2/3, P38MAPK and ERK1/2 activation induced by TGF-β1 at earlier time points. HTFs were treated with TGF-β1 (5 ng/ml) in the presence or absence of nintedanib (1 μM) for 15 min. p-Smad2/3 (A), p-P38MAPK and p-ERK1/2 (B) protein expression were analyzed by western blot and normalized to total Smad2/3, ERK1/2 or GAPDH, respectively. Data are presented as the means ± SD (n=3) of independent repeated experiments (* p<0.01; ** p<0.01;*** p<0.001; **** p<0.0001 compared to control or TGF-β1 group). To access the data, click or select the words “Appendix 2.”