**Figure 2. **Comparison of RGCs in four treated groups. **A**, **B**: Most retinal ganglion cells (RGCs) stained Fluoro-Gold (FG)-positive 7 days in the sham group and the sham plus echinacoside
(ECH) group. **C**: Many RGCs died, and activated microglia cells stained FG-positive after phagocytosis in the retinas of the rats that received
vehicle at 7 days after the ischemia/reperfusion (I/R) injury. **D**: In the retinas of the I/R plus ECH group, there were more RGCs stained FG-positive than in the retinas in the I/R plus vehicle
group. *p<0.05 versus sham group; ^{#}p<0.05 versus I/R plus vehicle group. **E**: The bar graph represents the total number of RGCs in all groups. There was a statistically significant loss, an approximate
31.11% reduction in the number of RGCs in the I/R plus vehicle group with respect to the I/R plus ECH group. Scale bar: 100
μm. *p<0.05.