Figure 4. Percentage of IR α-SMA and MLCp pixel counts in the TM of radial eye sections. Images are of defined areas centered on the
trabecular meshwork (TM) taken from sections of radially sectioned eyes that were immunohistochemically stained for alpha
smooth muscle action (α-SMA) and myosin light chain (MLC) p (MLCp; green). Samples were assessed from rats intracameral (IC) injected with TGF-β1+siRhoA for 7 days (A and E), TGF-β1+siEGFP for 7 days (B and F), TGF-β1+siRhoA for 35 days (C and G), TGF-β1+siEGFP for 35 days (D and H), TGF-β1 for 7 days (I and N), PBS for 7 days (J and O), TGF-β1 for 35 days (K and P), and PBS for 35 days (L and Q), as well as untreated Intact eyes (M and R). All images are representative of the eight images taken per retina from each treatment group (scale bar=50 µm). All sections
were nuclear counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). The percentage immunoreactivity (IR) pixel counts in the TM are presented for α-SMA (S) and MLCp (T) from the groups defined in A–R. Asterisks indicate the statistically significant differences at p<0.05 between the same treatments at 7 days and 35 days,
whereas the black lines indicate statistically significant differences (p<0.05) between groups at 7 days.