Table 1 of
Zhang, Mol Vis 2018; 24:700-711. Table 1 of
Zhang, Mol Vis 2018; 24:700-711.
Table 1. Primers information for the real-time quantitative polymerase chain reaction (PCR).
| Gene | Forward sequence (5′-3′) | Reverse sequence (5′-3′) | Products(bp) | Tm (°C) |
|---|---|---|---|---|
| CYP4V2 Exon1 | GTCTGGTCCTGAGCCTGC | AGGGCAGGGGTTTGGAAATA | 378 | 60 |
| CYP4V2 Exon2 | ACTGGTCACAACTTTCTCATCT | TTTGTGCTGAAATGGCTGAAA | 275 | 58 |
| CYP4V2 Exon3 | GGACTGAAAATGAACGACGGA | TCCAGCCTTTCTCCTCCTTT | 359 | 60 |
| CYP4V2 Exon4 | TCTCTTTCTCTCTCTCTCTCTGT | AGAAACAGATCGATGAGACTCAC | 273 | 58 |
| CYP4V2 Exon5 | CCGCTGCAAAATAAACACGAG | CCTTTACTGCTTAAACACATGCT | 300 | 58 |
| CYP4V2 Exon6 | CCCACGATTGCCTTCATCAA | CCACAATTGTAAAAGTCAGGGAC | 250 | 60 |
| CYP4V2 Exon8 | CAGTGCAGTCATCAAATCCAGA | GCCTTCCTGCTCATTACACTG | 300 | 58 |
| CYP4V2 Exon9 and Exon10 | CGTCCCGCTACAGTAGAAGA | CTCGGATCTCTGTGCAATGC | 284 | 58 |
| CYP4V2 Exon11 | CAGGACCTCTTGTTTCTCACA | GCGTTCATCTGCATTTCTCCT | 239 | 60 |
| GAPDH | AGAAGACTGTGGATGGCCC | TCAGTGTAGCCCAGGATGC | 280 | 60 |