Table 2 of Gooding, Mol Vis 2018; 24:690-699.

Table 2. Yield of RNA by different homogenization methods.

Tissue type Set screw TissueLyser Bullet blender Pellet pestle Dounce
Retina 4605 ± 1076 8345 ± 1450 5600 ± 481 2245 ± 452 7380 ± 994
Whole eye 7724 ± 2753 9784 ± 1779 8347 ± 834 2982 ± 1001 4584 ± 2103

Values are mean total ng in 40 μl eluent ± SD, n=8 for all retina measurements, n=10 for whole eyes except the pellet pestle (n=9). ANOVA multiple comparison analysis compared the mean of each technique within a tissue type (retina or whole eye) with the mean of every other technique for the same tissue. A full list of comparisons with p values is given in Appendix 1 and Appendix 2.

Gooding, Mol Vis 2018; 24:690-699. http://www.molvis.org/molvis/v24/690

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