(A) The amount of S-opsin mRNA was examined. The levels of mRNA after 6 h exposure to blue LED light and/or treated with bilberry extract of 10 μg/ml determined by real-time RT–PCR. Gapdh mRNA was used as the control. Data are the means ± SEMs (n=14). (B) The amount of S-opsin protein was examined. The levels of S-opsin after 6 h exposure to blue LED light and/or treated with bilberry extract of 10 μg/mL determined by immunoblot with or without cycloheximide. C: Control. B: Bilberry extract. V: Vehicle. To access the data, click or select the words “Appendix 2.”