Table 1 of Zelinka, Mol Vis 2018; 24:587-602.

Table 1. Frequency of and rhodopsin mutations recovered in F1 following gene targeting in zebrafish.

Target Transmitting G0 adults/No. tested Mutations recovered in F1 Allele DNA sequence Predicted Mutation
5′ rho, 2/5 (40%) 4 rhoWT GCCTATGTCCAATGCCACCGGGGTAGTC  
codon 17     rhofl6 GCCTATGTCCAATGCCTAACCCTAACCCGGGGTAGTC p.T17*
      rhofl7 GCCTATGTCCAAT—-CCGGGGGTAGTC p.A16_T17delinsP
      rhofl10 GCCTATGTCCAATG—CCGGGGTAGTC p.T17del
      - GCCTATGTCCA————-AGTC -
           
3′ rho, 3/7 (43%) 6 rhoWT ACCGCCTCCAAGACCGAGGCTTCGTCCG TGTCTTCCAGCTCCGTGTCTCCGGCGTAAA
codon 347     rhofl8 ACCAC-TGTCTGGA-CG—-AAGACCGAGGCTTCGTCCGTGTCTTCCAGCTCCGTGT p.A338_S339delinsTVWT
      rhofl9 ACCGCCTCCAAGACCGAGGCTTCGTCCGTGT————————AAA p.S347*
      - ACCGCCTCCAAGACCGAGGCTTCGTCCGT————————–AAA -
      - ACCGCCTCCAAGACCGAGGCTTCGTCCGTGTCTTCCAG——————– -
      - ACCG————AGGCTTCGTCCGTGTCTTCCAGCTCCGTGTCTCCGGCGTAAA -
      - ACCGCCTCCAAGACCGAGGCTTCGTCCG———————–GCGTAAA -

Sequence analysis and frequency of germ line transmission of 5′ rho and 3′ rho CRISPR/Cas9-induced alleles aligned to the wild-type rho allele (WT) show that the majority represents deletions and insertions (red text). Blue text represents CRISPR PAM sequence. Underlined DNA represents gRNA target sequence. Alleles characterized in this report are indicated by an allele designation.

Zelinka, Mol Vis 2018; 24:587-602. http://www.molvis.org/molvis/v24/587

©2018 Molecular Vision http://www.molvis.org/molvis/

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