Figure 4. Effect of antagonists on the expression of GABA receptors in the ARC in the chronic high IOP rat model. A: Immunofluorescence (IF) staining of GABA-A receptors and GABA-B receptors in brain slices at the location of the arcuate nucleus (ARC). B and C: Counting of positive cells in immunohistochemical (IHC)-stained sections with Image J (4 weeks + dimethyl sulfoxide (DMSO) versus 4 weeks + gabazine, *p<0.01; 4 weeks + DMSO versus 4 weeks + CGP55845, *p<0.01). D: IF staining of GABA-A receptors and GABA-B receptors in brain sections at the location of the ARC. E and F: Counting of positive cells in IF-stained sections with Image J (4 weeks + DMSO versus 4 weeks + gabazine, *p<0.01; 4 week + DMSO versus 4 weeks + CGP55845, *p<0.01). The data are expressed as mean ± standard error of the mean (SEM), n=6 per group. Bar=50 μm. 4w: chronic high intraocular pressure (IOP) rats without injection, 4w + DMSO: chronic high IOP rats with DMSO injection; 4 weeks + inhibitor: chronic high IOP rats with gabazine or CGP55845 injection.