Figure 1. The expression of Iba-1 and progranulin in the postnatal retina. A, B: Western blotting results show the expression of Iba-1 and progranulin during postnatal development. Typical bands and quantitative
data show the expression of Iba-1 and progranulin peaks at P7. C: Immunostaining of Iba-1 (green) and progranulin (magenta). Nuclei are stained with Hoechst 33342 (blue). Iba-1-positive
microglia increase at P7 and express progranulin. D, E: Western blotting results show the expression of retinal precursor cell markers and retinal cell markers. Retinal precursor
cell markers (nestin and Pax6) are decreased at P14 compared to P7. Retinal cell markers (rhodopsin, calbindin, and glutamine
synthetase [GS]) are increased at P14. These markers indicate photoreceptor cells, amacrine cells, and Müller glia, respectively.
Data are the mean ± standard error of the mean (SEM; n=4 to 6). ##p<0.01 versus P3 (Dunnett’s test). Scale bar=20 μm.