Figure 7 of Hollborn, Mol Vis 2018; 24:518-535.


Figure 7. Effects of knockdown of C9 with siRNA (left side) and administration of C9 protein (right side) on the expression levels of C9 (A, B), VEGF (C, E), and NLRP3 genes (F, G) and on the secretion of VEGF protein (D). Left side: The cells were transfected with nontargeted siRNA (siNon; 10 nM) and C9 siRNA (siC9; 10 nM). After 48 h, the cells were stimulated with a hyperosmotic medium (+ 100 mM NaCl) for 6 h or an isosmotic medium containing CoCl2 (150 µM) for 6 h or 24 h or were cultured for 6 h or 24 h in 1% O2 (as indicated by the panels of the bars). mRNA levels were determined with real-time RT–PCR analysis and are expressed as folds of unstimulated control. The level of VEGF-A165 protein in the cultured media was determined with ELISA and is expressed in percent of unstimulated control (100%). Right side: The cells were stimulated with a hyperosmotic medium (+ 100 mM NaCl) or an isosmotic medium containing CoCl2 (150 µM) for 6 h and 24 h in the absence (control) and presence of C9 protein (0.1 and 10 nM, respectively). The numbers of independent experiments using cell lines from different donors are indicated in the bars. Significant difference versus unstimulated control: *p<0.05. Significant difference versus siNon: ●p<0.05. Significant difference versus NaCl and CoCl2 control: ○p<0.05.